The epithelial cell response to health and disease associated oral biofilm models

Ramage, G. , Lappin, D.F., Millhouse, E., Malcolm, J. , Jose, A., Yang, J., Bradshaw, D.J., Pratten, J.R. and Culshaw, S. (2016) The epithelial cell response to health and disease associated oral biofilm models. Journal of Periodontal Research, 52(3), pp. 325-333. (doi: 10.1111/jre.12395) (PMID:27330034) (PMCID:PMC5412879)

Abstract

Background and Objective: Different bacteria differentially stimulate epithelial cells. Biofilm composition and viability are likely to influence the epithelial response. In vitro model systems are commonly used to investigate periodontitis-associated bacteria and their interactions with the host; therefore, understanding factors that influence biofilm–cell interactions is essential. The present study aimed to develop in vitro monospecies and multispecies biofilms and investigate the epithelial response to these biofilms. Material and Methods: Bacterial biofilms were cultured in vitro and then either live or methanol-fixed biofilms were co-cultured with epithelial cells. Changes in epithelial cell viability, gene expression and cytokine content of culture supernatants were evaluated. Results: Bacterial viability was better preserved within mixed-species biofilm culture than within single-species biofilm culture. Both mixed- and single-species biofilms stimulated increased expression of mRNA for interleukin 8 (IL8), C-X-C motif chemokine ligand 3 (CXCL3), C-X-C motif chemokine ligand 1 (CXCL1), interleukin 1 (IL1), interleukin 6 (IL6), colony-stimulating factor 2 (CSF2) and tumour necrosis factor (TNF), and the response was greatest in response to mixed-species biofilms. Following co-culture, cytokines detected in the supernatants included IL-8, IL-6, granulocyte colony-stimulating factor and granulocyte–macrophage colony-stimulating factor, with the greatest release of cytokines found following co-culture with methanol-fixed, mixed-species biofilms. Conclusions: These data show that epithelial cells generate a distinct cytokine gene- and protein-expression signature in response to live or fixed, single- or multispecies biofilms.

• Item Type: Articles
• Additional Information: EM was funded through a BBSRC CASE studentship (BB/J500318/1)
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Millhouse, Dr Emma and Lappin, Dr David and Jose, Mr Anto and Ramage, Professor Gordon and Malcolm, Dr Jennifer and Culshaw, Professor Shauna
• Authors: Ramage, G., Lappin, D.F., Millhouse, E., Malcolm, J., Jose, A., Yang, J., Bradshaw, D.J., Pratten, J.R., and Culshaw, S.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
• Journal Name: Journal of Periodontal Research
• Publisher: Wiley
• ISSN: 0022-3484
• ISSN (Online): 1600-0765
• Published Online: 22 June 2016
• Copyright Holders: Copyright © 2016 The Authors
• First Published: First published in Journal of Periodontal Research 52(3):325–333
• Publisher Policy: Reproduced under a Creative Commons License