The use of telomere biology to identify and develop superior nitrone based anti-oxidants

Sklavounou, E., Hay, A., Ashraf, N., Lamb, K., Brown, E., Mac Intyre, A., George, W.D., Hartley, R.C. and Shiels, P.G. (2006) The use of telomere biology to identify and develop superior nitrone based anti-oxidants. Biochemical and Biophysical Research Communications, 347, pp. 420-427. (doi: 10.1016/j.bbrc.2006.06.087)

Abstract

We have employed a biological chemistry approach to dissect the mechanisms underpinning cellular responses to oxidant stress and to develop biologically relevant anti-oxidants. We have used telomere biology to define cellular stress responses and have observed telomere independent, <i>p</i>21- and <i>p</i>16-dependent stasis following oxidative insult in human fibroblasts. This was accompanied by reduction in XRCC5 expression and elevation of SIRT 1 expression. Using these markers in conjunction with senescence-associated β-galactosidase expression, we have developed and screened novel nitrone based anti-oxidant compounds. We have identified functional compounds that are unsuitable for use in primary human cells. This has allowed subsequent identification of suitably structured compounds that act as superior biological anti-oxidants, which have potential for use in clinical interventions.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hartley, Professor Richard and Shiels, Professor Paul
• Authors: Sklavounou, E., Hay, A., Ashraf, N., Lamb, K., Brown, E., Mac Intyre, A., George, W.D., Hartley, R.C., and Shiels, P.G.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Biochemical and Biophysical Research Communications
• ISSN: 0006-291X