A robust lithographic method for multiplex surface pattering

Cuestas-Ayllon, C., Xiao, Q., Glidle, A., Riehle, M.O. , Cooper, J.M. , de la Fuente, J.M. and Yin, H.B. (2012) A robust lithographic method for multiplex surface pattering. Current Analytical Chemistry, 9(1), pp. 29-36. (doi: 10.2174/1573411011309010029)

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Surface engineering provides a powerful tool to create model systems to investigate the complex interactions between cells and the extracellular matrix (ECM). Cell adhesive peptide ligands (e.g. Arginine-Glycine-Aspatate (RGD) sequence) and carbohydrates are abundant in the ECM, and both are involved in mediating cell adhesion. However, how these interact is not well understood in vivo. Model systems to investigate their collective roles are rare. We have developed a robust surface patterning method allowing spatial control of carbohydrate (i.e. mannose) and RGD on glass while overcoming non-specific biomolecular interactions. The method effectively combines a traditional photolithographic process with two robust chemical pathways, namely click chemistry and silanization, in order to tailor the surface chemistry. This method is especially promising for the quantitative immobilization of different molecules and holds potential for the investigation of interactions between carbohydrates and common ECM proteins on cell adhesion. - See more at: http://www.eurekaselect.com/105477/article#sthash.LKEggb6P.dpuf

Item Type:Articles
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing and Cooper, Professor Jonathan and Riehle, Dr Mathis and Glidle, Dr Andrew
Authors: Cuestas-Ayllon, C., Xiao, Q., Glidle, A., Riehle, M.O., Cooper, J.M., de la Fuente, J.M., and Yin, H.B.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Current Analytical Chemistry
Publisher:Bentham Science Publishers
ISSN (Online):1875-6727

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