Analysis of osteoclastogenesis/osteoblastogenesis on nanotopographical titania surfaces

Silverwood, R. K. et al. (2016) Analysis of osteoclastogenesis/osteoblastogenesis on nanotopographical titania surfaces. Advanced Healthcare Materials, 5(8), pp. 947-955. (doi: 10.1002/adhm.201500664) (PMID:26890261)

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A focus of orthopedic research is to improve osteointegration and outcomes of joint replacement. Material surface topography has been shown to alter cell adhesion, proliferation, and growth. The use of nanotopographical features to promote cell adhesion and bone formation is hoped to improve osteointegration and clinical outcomes. Use of block-copolymer self-assem-bled nanopatterns allows nanopillars to form via templated anodization with control over height and order, which has been shown to be of cellular importance. This project assesses the outcome of a human bone marrow-derived co-culture of adherent osteoprogenitors and osteoclast progenitors on polished titania and titania patterned with 15 nm nanopillars, fabricated by a block-copolymer templated anodization technique. Substrate implantation in rabbit femurs is performed to confirm the in vivo bone/implant integration. Quantitative and qualitative results demonstrate increased osteogenesis on the nanopillar substrate with scanning electron microscopy, histochemical staining, and real-time quantitative reverse-transcription polymerase chain reaction analysis performed. Osteoblast/osteoclast co-culture analysis shows an increase in osteoblastogenesis-related gene expression and reduction in osteoclastogenesis. Supporting this in vitro finding, in vivo implantation of substrates in rabbit femora indicates increased implant/bone contact by ≈20%. These favorable osteogenic characteristics demonstrate the potential of 15 nm titania nanopillars fabricated by the block-copolymer templated anodization technique.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Tsimbouri, Dr Monica and Fairhurst, Dr Paul Gilbert and Dalby, Professor Matthew
Authors: Silverwood, R. K., Fairhurst, P. G., Sjostrom, T., Welsh, F., Sun, Y., Li, G., Yu, B., Young, P. S., Su, B., Meek, R. M.D., Dalby, M. J., and Tsimbouri, P.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Advanced Healthcare Materials
ISSN (Online):2192-2659
Published Online:18 February 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Advanced Healthcare Materials 5(8):947-955
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.211

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
615571Multiscale topographical modulation of cells and bacteria for next generation orthopaedic implantsMatthew DalbyEngineering & Physical Sciences Research Council (EPSRC)EP/K034898/1RI MOLECULAR CELL & SYSTEMS BIOLOGY