Effect of pre-analytical treatments on bovine milk acute phase proteins

Thomas, F. C., Santana, A. M., Waterston, M., Haining, H. and Eckersall, P. D. (2016) Effect of pre-analytical treatments on bovine milk acute phase proteins. Veterinary Research, 12, 151. (doi: 10.1186/s12917-016-0769-6) (PMID:27457305) (PMCID:PMC4960790)

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Background Samples for diagnostic procedures often require some form of pre-analytical preparation for preservation or safe handling during transportation prior to analysis in the laboratory. This is particularly important for milk samples which frequently need preservatives to retain milk composition as close to that found in freshly collected samples as possible. Methods Milk samples were treated by heating at 56 °C for 30 min or preserved by addition of either potassium dichromate or bronopol respectively. Haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were measured in the various treatment groups and in control samples which were not treated, using enzyme linked immunoassays. The concentrations of each APP were compared between treated and non-treated groups using the Wilcoxon signed ranks tests. Results Heat treatment of samples was found to have a significant lowering effect on milk M-SAA3 and CRP but not Hp. The use of potassium dichromate and bronopol as preservatives in milk had no significant effects on milk Hp and M-SAA3 concentration but lowered milk CRP values compared to controls. Conclusions The observed effects of heating and preservative use on milk APP should be taken into consideration when assaying samples which have undergone heat treatment as a result of international transfer regulations involving biological samples or samples needing chemical preservation prior to transport to laboratory.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Eckersall, Professor David and Santana, Dr Andre and Haining, Dr Hayley and Waterston, Miss Mary
Authors: Thomas, F. C., Santana, A. M., Waterston, M., Haining, H., and Eckersall, P. D.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Veterinary Research
Publisher:BioMed Central Ltd.
ISSN (Online):1297-9716
Published Online:25 July 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Veterinary Research 12:151
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.319

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
683931Acute phase protein as biomarkers of disease in livestock and aquaculturePeter EckersallBiotechnology and Biological Sciences Research Council (BBSRC)BB/M022021/1RI BIODIVERSITY ANIMAL HEALTH & COMPMED