Bailey, M. et al. (2016) Stability of small ubiquitin-like modifier (SUMO) proteases OVERLY TOLERANT TO SALT1 and -2 modulates salicylic acid signalling and SUMO1/2 conjugation inArabidopsis thaliana. Journal of Experimental Botany, 67(1), pp. 353-363. (doi: 10.1093/jxb/erv468) (PMID:26494731) (PMCID:PMC4682439)
|
Text
115902.pdf - Published Version Available under License Creative Commons Attribution. 3MB |
Abstract
Small ubiquitin-like modifier proteases 1 and 2 (SUMO1/2) have been linked to the regulation of salicylic acid (SA)-mediated defence signalling in Arabidopsis thaliana. In order to define the role of the SUMO proteases OVERLY TOLERANT TO SALT1 and -2 (OTS1/2) in defence and to provide insight into SUMO1/2-mediated regulation of SA signalling, we examined the status of SA-mediated defences in ots1/2 mutants. The ots1 ots2 double mutant displayed enhanced resistance to virulent Pseudomonas syringae and higher levels of SA compared with wild-type (WT) plants. Furthermore, ots1 ots2 mutants exhibited upregulated expression of the SA biosynthesis gene ICS1 in addition to enhanced SA-responsive ICS1 expression beyond that of WT. SA stimulated OTS1/2 degradation and promoted accumulation of SUMO1/2 conjugates. These results indicate that OTS1 and -2 act in a feedback loop in SA signalling and that de novo OTS1/2 synthesis works antagonistically to SA-promoted degradation, adjusting the abundance of OTS1/2 to moderate SA signalling. Accumulation of SUMO1/2 conjugates coincides with SA-promoted OTS degradation and may play a positive role in SA-mediated signalling in addition to its repressive roles reported elsewhere.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Conti, Dr Lucio and Milner, Dr Joel and Sadanandom, Dr Ari and Love, Dr Andrew |
| Authors: | Bailey, M., Srivastava, A., Conti, L., Nelis, S., Zhang, C., Florance, H., Love, A., Milner, J., Napier, R., Grant, M., and Sadanandom, A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Life Sciences |
| Journal Name: | Journal of Experimental Botany |
| Publisher: | Oxford University Press |
| ISSN: | 0022-0957 |
| ISSN (Online): | 1460-2431 |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information