Henry, S. L., Withers, J. M., Singh, I., Cooper, J. M. , Clark, A. W. , Burley, G. A. and Cogdell, R. J. (2016) DNA-directed spatial assembly of photosynthetic light-harvesting proteins. Organic and Biomolecular Chemistry, 14(4), pp. 1359-1362. (doi: 10.1039/c5ob02351c) (PMID:26660647)
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Abstract
This manuscript describes the surface immobilization of a light-harvesting complex to prescribed locations directed by the sequence-selective recognition of duplex DNA. An engineered light-harvesting complex (RC-LH1) derived from Rhodopseudomonas (Rps.) palustris containing the zinc finger (ZF) domain zif268 was prepared. The zif268 domain directed the binding of zfRC-LH1 to target double-stranded DNA sequences both in solution and when immobilized on lithographically defined micro-patterns. Excitation energy transfer from the carotenoids to the bacteriochlorophyll pigments within zfRC-LH1 confirmed that the functional and structural integrity of the complex is retained after surface immobilization.
| Item Type: | Articles |
|---|---|
| Additional Information: | SLH and RJC were supported by funding from Human Frontier of Science Program. AWC was supported by the Royal Academy of Engineering (grant number 10216/103). This research was supported as a part of the Photosynthetic Antenna Research Centre (PARC), an Energy Frontier Research Centre funded by the DOE, Office of Science, Office of Basic Energy Sciences under Award Number DE-SC 0001035. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Henry, Dr Sarah and Burley, Dr Glenn and Cooper, Professor Jonathan and Cogdell, Professor Richard and Clark, Professor Alasdair |
| Authors: | Henry, S. L., Withers, J. M., Singh, I., Cooper, J. M., Clark, A. W., Burley, G. A., and Cogdell, R. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Organic and Biomolecular Chemistry |
| Journal Abbr.: | Org. Biomol. Chem. |
| Publisher: | Royal Society of Chemistry |
| ISSN: | 1477-0520 |
| ISSN (Online): | 1477-0539 |
| Copyright Holders: | Copyright © 2016 Royal Society of Chemistry |
| First Published: | First published in Organic and Biomolecular Chemistry 14(4):1359-1362 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher. |
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