Species and strain comparisons in the macromolecular binding of extremely low doses of [14C]benzene in rodents, using accelerator mass spectrometry

Mani, C., Freeman, S. , Nelson, D. O., Vogel, J. S. and Turteltaub, K. W. (1999) Species and strain comparisons in the macromolecular binding of extremely low doses of [14C]benzene in rodents, using accelerator mass spectrometry. Toxicology and Applied Pharmacology, 159(2), pp. 83-90. (doi:10.1006/taap.1999.8707) (PMID:10495771)

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Abstract

The kinetics of macromolecular binding of a 5 μg/kg body wt dose of [14C]benzene was studied over 48 h in B6C3F1, DBA/2, and C57BL/6 mice and Fischer rats to determine if adduct levels reflect known differences in metabolic capacity, genotoxicity, and carcinogenic potency. Previous studies have suggested that differences in benzene toxicity among strains result from differences in metabolism. Rats and mice were administered [14C]benzene (i.p.), followed by removal of liver and bone marrow at time intervals up to 48 h postexposure. Protein and DNA were isolated and analyzed by accelerator mass spectrometry. Area under the curves for protein and DNA adducts in bone marrow were greatest in B6C3F1 mouse > DBA/2 mouse > C57BL/6 mouse > Fischer rat. These data are consistent with the hypothesis that metabolic capacity contributes to the difference in benzene's carcinogenicity among species. Additionally, these data suggest that target organ adduct levels correlate with tumorigenicity and thus may be indicative of an individuals risk.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Freeman, Professor Stewart
Authors: Mani, C., Freeman, S., Nelson, D. O., Vogel, J. S., and Turteltaub, K. W.
College/School:College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Toxicology and Applied Pharmacology
Publisher:Elsevier
ISSN:0041-008X
ISSN (Online):1096-0333

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