Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine

Gilman, S. D., Gee, S. J., Hammock, B. D., Vogel, J. S., Haack, K., Buchholz, B. A., Freeman, S. P.H.T. , Wester, R. C., Hui, X. and Maibach, H. I. (1998) Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine. Analytical Chemistry, 70(16), pp. 3463-3469. (doi: 10.1021/ac971383v) (PMID:9726169)

Abstract

Accelerator mass spectrometry (AMS) has been applied to the detection of 14C-labeled urinary metabolites of the triazine herbicide, atrazine, and the analytical performance of AMS has been directly compared to that of liquid scintillation counting (LSC). Ten human subjects were given a dermal dose of 14C-labeled atrazine over 24 h, and urine from the subjects was collected over a 7-day period. Concentrations of 14C in the samples have been determined by AMS and LSC and range from 1.8 fmol/mL to 4.3 pmol/mL. Data from these two methods have a correlation coefficient of 0.998 for a linear plot of the entire sample set. Accelerator mass spectrometry provides superior concentration (2.2 vs 27 fmol/mL) and mass (5.5 vs 54 000 amol) detection limits relative to those of LSC for these samples. The precision of the data provided by AMS for low-level samples is 1.7%, and the day-to-day reproducibility of the AMS measurements is 3.9%. Factors limiting AMS detection limits for these samples and ways in which these can be improved are examined.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Freeman, Professor Stewart
• Authors: Gilman, S. D., Gee, S. J., Hammock, B. D., Vogel, J. S., Haack, K., Buchholz, B. A., Freeman, S. P.H.T., Wester, R. C., Hui, X., and Maibach, H. I.
• College/School: College of Science and Engineering > Scottish Universities Environmental Research Centre
• Journal Name: Analytical Chemistry
• Publisher: American Chemical Society
• ISSN: 0003-2700
• ISSN (Online): 1520-6882