Gliding associated proteins play essential roles during the formation of the inner membrane complex of toxoplasma gondii

Harding, C. R. , Egarter, S., Gow, M., Jiménez-Ruiz, E. , Ferguson, D. J.P. and Meissner, M. (2016) Gliding associated proteins play essential roles during the formation of the inner membrane complex of toxoplasma gondii. PLoS Pathogens, 12(2), e1005403. (doi: 10.1371/journal.ppat.1005403) (PMID:26845335) (PMCID:PMC4742064)

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The inner membrane complex (IMC) of apicomplexan parasites is a specialised structure localised beneath the parasite’s plasma membrane, and is important for parasite stability and intracellular replication. Furthermore, it serves as an anchor for the myosin A motor complex, termed the glideosome. While the role of this protein complex in parasite motility and host cell invasion has been well described, additional roles during the asexual life cycle are unknown. Here, we demonstrate that core elements of the glideosome, the gliding associated proteins GAP40 and GAP50 as well as members of the GAPM family, have critical roles in the biogenesis of the IMC during intracellular replication. Deletion or disruption of these genes resulted in the rapid collapse of developing parasites after initiation of the cell cycle and led to redistribution of other glideosome components.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Meissner, Professor Markus and Jimenez Ruiz, Dr Elena and Harding, Dr Clare and Egarter, Ms Saskia and Gow, Mr Matthew
Authors: Harding, C. R., Egarter, S., Gow, M., Jiménez-Ruiz, E., Ferguson, D. J.P., and Meissner, M.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:PLoS Pathogens
Publisher:Public Library of Science
ISSN (Online):1553-7374
Copyright Holders:Copyright © 2016 Harding et al.
First Published:First published in PLoS Pathogens 12(2):e1005403
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
659231Developing insights into Toxoplasma gondii pathogenesis through analysis of both parasite and host cell proteins required for the parasite's lifecycleClare HardingWellcome Trust (WELLCOME)103972/Z/14/ZIII - PARASITOLOGY
501601Systematic analysis of essential parasite genes linked to invasion of the host cell in Toxoplasma gondiiMarkus MeissnerWellcome Trust (WELLCOME)087582/Z/08/ZIII - PARASITOLOGY
371798The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/B/08/ZIII - PARASITOLOGY