Nelson, S. M. , Pastuszek, E., Kloss, G., Malinowska, I., Liss, J., Lukaszuk, A., Plociennik, L. and Lukaszuk, K. (2015) Two new automated, compared with two enzyme-linked immunosorbent, antimüllerian hormone assays. Fertility and Sterility, 104(4), 1016-1021.e6. (doi: 10.1016/j.fertnstert.2015.06.024) (PMID:26183313)
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Abstract
Objective: To compare new automated antimüllerian hormone (AMH) assay performance characteristics from the new automated Elecsys AMH (Roche; Elecsys) and Access AMH (Beckman Coulter; Access) assays with the existing AMH Gen II ELISA (enzyme-linked immunosorbent assay; Gen II; Beckman Coulter) and AMH ELISA (Ansh Labs) assays. Design: Prospective assay evaluation. Setting: University-affiliated clinical chemistry laboratory. Patient(s): Patients referred for serum AMH measurement (n = 83) before start of in vitro fertilization cycle between September 2014 and October 2014. Intervention(s): None. Main Outcome Measure(s): Serum AMH concentration.: Result(s): Intra-assay coefficients of variation were low; Ansh ≤ 9.0%; Gen II ≤ 5.8%; Access ≤ 10.7%; and Elecsys ≤ 2.8%. The Passing-Bablok regression equations (pmol/L) were y (Access) = 0.128 + (0.781 × Gen II); and y (Access) = 0.302 + (0.742 x Ansh). For y (Elecys) = 0.087 + (0.729 x Gen II) and y (Elecys) = 0.253 + (0.688 x Ansh Labs). For y (Elecys) = 0.943 − (0.037 × Access). For all the assays, AMH exhibited a moderate positive correlation with AFC (r = 0.62–0.64); number of cumulus oocyte complexes (r = 0.60–0.64); and metaphase II oocytes (r = 0.48–0.50). Accuracy of pregnancy prediction, as determined by area under the receiver operating characteristic curve, was uniformly low for all assays (0.62–0.63). Conclusion(s): The novel automated assays exhibit strong concordance in calibration, but derived values are substantially lower than those obtained from pre-existing assays, with assay-specific interpretation required for routine clinical use. These results highlight the need for an international standard of measurement of AMH.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Nelson, Professor Scott |
Authors: | Nelson, S. M., Pastuszek, E., Kloss, G., Malinowska, I., Liss, J., Lukaszuk, A., Plociennik, L., and Lukaszuk, K. |
College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
Journal Name: | Fertility and Sterility |
Publisher: | Elsevier |
ISSN: | 0015-0282 |
ISSN (Online): | 1556-5653 |
Copyright Holders: | Copyright © 2015 Elsevier |
First Published: | First published in Fertility and Sterility 104(4):1016-1021.e6 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher. |
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