Kalafatakis, K., Gkanti, V., Mackenzie-Gray Scott, C. A., Zarros, A. , Baillie, G. S. and Tsakiris, S. (2015) Acetylcholinesterase activity as a neurotoxicity marker within the context of experimentally-simulated hyperprolinaemia: an in vitro approach. Journal of Natural Science, Biology and Medicine, 6(3), pp. 98-101. (doi: 10.4103/0976-9668.166099)
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Abstract
Hyperprolinaemia is characterized by increased tissue accumulation of proline (Pro) and is known to exert serious cognitive and/or neuropsychiatric symptomatology as a direct result of Pro accumulation in the brain. The aim of this study was to explore a putative link between experimentally-simulated hyperprolinaemia and the activity of acetylcholinesterase (AChE); a crucial neurotoxicity marker. In vitro experiments were undertaken on purified eel-derived AChE, as well as on adult mouse brain homogenates, in order to examine the effect of a spectrum of Pro concentrations (3, 30, 500, and 1000 μM) on this marker. Our data showed that although Pro exerted a significant inhibitory effect on pure AChE activity, mouse brain-derived membrane-bound AChE activity was found either unaltered or significantly increased following incubation with Pro. The use of AChE activity as a neurotoxicity marker within the context of experimentally-simulated hyperprolinaemia should be considered with caution and in parallel with a number of other experimental parameters.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Baillie, Professor George and Zarros, Dr Apostolos |
| Authors: | Kalafatakis, K., Gkanti, V., Mackenzie-Gray Scott, C. A., Zarros, A., Baillie, G. S., and Tsakiris, S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Journal Name: | Journal of Natural Science, Biology and Medicine |
| Publisher: | Wolters Kluwer |
| ISSN: | 0976-9668 |
| ISSN (Online): | 2229-7707 |
| Copyright Holders: | Copyright © 2015 Journal of Natural Science, Biology and Medicine |
| First Published: | First published in Journal of Natural Science, Biology and Medicine 6(3):98-101 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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