Tullius, R., Karimullah, A. S. , Rodier, M., Fitzpatrick, B., Gadegaard, N. , Barron, L. D., Rotello, V. M., Cooke, G. , Lapthorn, A. and Kadodwala, M. (2015) "Superchiral” spectroscopy: detection of protein higher order hierarchical structure with chiral plasmonic nanostructures. Journal of the American Chemical Society, 137(26), pp. 8380-8383. (doi: 10.1021/jacs.5b04806) (PMID:26102606)
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Abstract
Optical spectroscopic methods do not routinely provide information on higher order hierarchical structure (tertiary/quaternary) of biological macromolecules and assemblies. This necessitates the use of time-consuming and material intensive techniques, such as protein crystallography, NMR, and electron microscopy. Here we demonstrate a spectroscopic phenomenon, superchiral polarimetry, which can rapidly characterize ligand-induced changes in protein higher order (tertiary/quaternary) structure at the picogram level, which is undetectable using conventional CD spectroscopy. This is achieved by utilizing the enhanced sensitivity of superchiral evanescent fields to mesoscale chiral structure.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Lapthorn, Dr Adrian and Kadodwala, Professor Malcolm and Cooke, Professor Graeme and TULLIUS, Ryan and Karimullah, Dr Affar and Barron, Professor Laurence and Fitzpatrick, Dr Brian and Gadegaard, Professor Nikolaj |
Authors: | Tullius, R., Karimullah, A. S., Rodier, M., Fitzpatrick, B., Gadegaard, N., Barron, L. D., Rotello, V. M., Cooke, G., Lapthorn, A., and Kadodwala, M. |
College/School: | College of Science and Engineering > School of Chemistry College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | Journal of the American Chemical Society |
Publisher: | American Chemical Society |
ISSN: | 0002-7863 |
ISSN (Online): | 1520-5126 |
Copyright Holders: | Copyright © 2015 American Chemical Society |
First Published: | First published in Journal of the American Chemical Society 137(26):8380-8383 |
Publisher Policy: | Reproduced under a Creative Commons License |
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