Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples

Tveen-Jensen, K., Gesellchen, F., Wilson, R. , Spickett, C. M., Cooper, J. M. and Pitt, A. R. (2015) Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples. Scientific Reports, 5, 9736. (doi: 10.1038/srep09736) (PMID:25978651) (PMCID:PMC4432867)

Abstract

Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Gesellchen, Dr Frank and Wilson, Dr Robert and Cooper, Professor Jonathan
• Authors: Tveen-Jensen, K., Gesellchen, F., Wilson, R., Spickett, C. M., Cooper, J. M., and Pitt, A. R.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Scientific Reports
• Publisher: Nature Publishing Group
• ISSN: 2045-2322
• ISSN (Online): 2045-2322
• Copyright Holders: Copyright © 2015 The Authors
• First Published: First published Scientific Reports 5:9736
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
55352	1	Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing	Jonathan Cooper	Engineering & Physical Sciences Research Council (EPSRC)	EP/I017887/1	ENG - BIOMEDICAL ENGINEERING
61702	1	Advanced Diagnostics using Phononics	Jonathan Cooper	Engineering & Physical Sciences Research Council (EPSRC)	EP/K027611/1	ENG - BIOMEDICAL ENGINEERING