Fluorescence kinetics of tryptophan in a heterogeneous environment

Rolinski, O. J. and Vyshemirsky, V. (2014) Fluorescence kinetics of tryptophan in a heterogeneous environment. Methods and Applications in Fluorescence, 2(4), 045002. (doi: 10.1088/2050-6120/2/4/045002)

Full text not currently available from Enlighten.


The potentially highly informative, but complex fluorescence decay of amino acids in protein is not fully understood and presents a barrier to understanding. Here we have tested a new and general approach to describing experimentally measured the fluorescence decay in a heterogeneous macroscopic sample. The decay parameters carry information on the features of the kinetics induced by the environment's heterogeneity. Bayesian interference demonstrated that the model fits well to the fluorescence decay of tryptophan in human serum albumin (HSA). The approach has the potential to accelerate photophysical research of heterogeneous media and, specifically, to solve a critical outstanding problem in interpreting protein fluorescence, paving the way to further progress in biomedical research.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Vyshemirsky, Dr Vladislav
Authors: Rolinski, O. J., and Vyshemirsky, V.
College/School:College of Science and Engineering > School of Mathematics and Statistics > Statistics
Journal Name:Methods and Applications in Fluorescence
Publisher:IOP Publishing
ISSN (Online):2050-6120

University Staff: Request a correction | Enlighten Editors: Update this record