Analysis of novel imidazoles from isolated perfused rabbit heart by two high-performance liquid chromatographic methods

O'Dowd, A., O'Dowd, J.J., O'Dowd, J.J., MacFarlane, N., Abe, H. and Miller, D.J. (1992) Analysis of novel imidazoles from isolated perfused rabbit heart by two high-performance liquid chromatographic methods. Journal of Chromatography B: Biomedical Sciences and Applications, 577(2), pp. 347-53. (doi:10.1016/0378-4347(92)80257-Q) (PMID:1400766)

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Abstract

The paper reports two analytical high-performance liquid chromatographic methods to detect and quantify cardiac-derived histidyl derivatives. Method A relies on relative hydrophobicities as a basis of separation. Method B is an ion-pairing method in which the compounds are eluted in an entirely different order. Fractions collected from method A have been co-eluted in admixture in method B with authentic reference compounds. Thus the existence of the following imidazole ring-containing compounds derived from heart have been confirmed: N-acetylhistidine, N-acetyl-1-methylhistidine, N-acetylcarnosine, N-acetylhomocarnosine, homocarnosine, anserine, carnosine, balenine. Compounds were found in both tissue samples and perfusates.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:MacFarlane, Professor Niall and O'Dowd, Dr John
Authors: O'Dowd, A., O'Dowd, J.J., O'Dowd, J.J., MacFarlane, N., Abe, H., and Miller, D.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Health and Wellbeing > Robertson Centre
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Journal of Chromatography B: Biomedical Sciences and Applications
Publisher:Elsevier
ISSN:1387-2273
ISSN (Online):1872-812X

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