Buckley, A. M., Petersen, J., Roe, A. J. , Douce, G. R. and Christie, J. M. (2015) LOV-based reporters for fluorescence imaging. Current Opinion in Chemical Biology, 27, pp. 39-45. (doi: 10.1016/j.cbpa.2015.05.011) (PMID:26087123)
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Abstract
Chromophore-binding domains from plant and bacterial photoreceptor proteins have recently gathered increasing attention as new sources of genetically encoded fluorescent proteins (FPs). In particular, FPs based on the flavin-binding LOV (light, oxygen, or voltage sensing) domain offer advantages over green fluorescent protein (GFP) owing to their smaller size, pH and thermal stability, utility under anaerobic conditions and their ability to generate reactive oxygen species. This review focuses on the potential applications of this emerging class of fluorescent reporters, discusses the advantages and limitations of LOV-based FPs, whilst offering insights regarding the further development of this technology for bioimaging and photodynamic therapy.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Roe, Professor Andrew and Petersen, Dr Jan and Douce, Dr Gillian and Buckley, Dr Anthony and Christie, Professor John |
| Authors: | Buckley, A. M., Petersen, J., Roe, A. J., Douce, G. R., and Christie, J. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Current Opinion in Chemical Biology |
| Publisher: | Elsevier Ltd., Current Opinion Journals |
| ISSN: | 1367-5931 |
| ISSN (Online): | 1879-0402 |
| Copyright Holders: | Copyright © 2015 The Authors |
| First Published: | First published in Current Opinion in Chemical Biology |
| Publisher Policy: | Reproduced under a Creative Commons License 27:39-45 |
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