Sadler, J. B.A., Bryant, N. J. and Gould, G. W. (2015) Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking. Molecular Biology of the Cell, 26(3), pp. 530-536. (doi: 10.1091/mbc.E14-09-1368) (PMID:25501368)
Full text not currently available from Enlighten.
Abstract
The fusion of GLUT4-containing vesicles with the plasma membrane of adipocytes is a key facet of insulin action. This process is mediated by the formation of functional soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) complexes between the plasma membrane t-SNARE complex and the vesicle v-SNARE or VAMP. The t-SNARE complex consists of Syntaxin4 and SNAP23, and whereas many studies identify VAMP2 as the v-SNARE, others suggest that either VAMP3 or VAMP8 may also fulfil this role. Here we characterized the levels of expression, distribution, and association of all the VAMPs expressed in 3T3-L1 adipocytes to provide the first systematic analysis of all members of this protein family for any cell type. Despite our finding that all VAMP isoforms form SDS-resistant SNARE complexes with Syntaxin4/SNAP23 in vitro, a combination of levels of expression (which vary by >30-fold), subcellular distribution, and coimmunoprecipitation analyses lead us to propose that VAMP2 is the major v-SNARE involved in GLUT4 trafficking to the surface of 3T3-L1 adipocytes.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Sadler, Miss Jessica and Gould, Professor Gwyn and Bryant, Dr Nia |
Authors: | Sadler, J. B.A., Bryant, N. J., and Gould, G. W. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Molecular Biology of the Cell |
Publisher: | American Society for Cell Biology |
ISSN: | 1059-1524 |
ISSN (Online): | 1939-4586 |
University Staff: Request a correction | Enlighten Editors: Update this record