mik1⁺ G1-S transcription regulates mitotic entry in fission yeast

Ng, S.S., Anderson, M., White, S. and McInerny, C.J. (2001) mik1⁺ G1-S transcription regulates mitotic entry in fission yeast. FEBS Letters, 503(2/3), pp. 131-134. (doi: 10.1016/S0014-5793(01)02720-X)

Abstract

In the fission yeast Schizosaccharomyces pombe Mik1p, in combination with Wee1p, is an important inhibitor of mitosis through direct phosphorylation of Cdc2p. Here we present the observation that mik1⁺ is transcribed during G1- and S-phase in normally dividing cells. mik1⁺ transcription is regulated by the MCB–DSC1 system, which controls expression of other genes at the G1–S interval. mik1⁺ is shown to be an important target of MCB–DSC1 as it is epistatic for the mitotic delay phenotype displayed in cdc10-C4 cells, which are mutated in a component of DSC1. The mitotic delay in cdc10-C4 cells is bypassed by cdc2-1w, suggesting that mik1⁺ acts directly on cdc2⁺, with no checkpoint function involved. Thus, mik1⁺ represents a new type of MCB–DSC1 regulated gene in fission yeast, whose gene product is exclusively expressed during G1- and S-phase to prevent premature mitosis during this cell cycle stage.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McInerny, Dr Chris and White, Dr Stewart
• Authors: Ng, S.S., Anderson, M., White, S., and McInerny, C.J.
• College/School: College of Medical Veterinary and Life Sciences > School of Life Sciences; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: FEBS Letters
• ISSN: 0014-5793
• ISSN (Online): 1873-3468