Hu, Z., Glidle, A., Ironside, C., Cooper, J. M. and Yin, H. (2015) An integrated microspectrometer for localised multiplexing measurements. Lab on a Chip, 15(1), pp. 283-289. (doi: 10.1039/C4LC00952E)
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Publisher's URL: http://dx.doi.org/10.1039/C4LC00952E
Abstract
We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel “bead–AWG” platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Yin, Professor Huabing and Cooper, Professor Jonathan and Ironside, Professor Charles and Hu, Mr Zhixiong and Glidle, Dr Andrew |
| Authors: | Hu, Z., Glidle, A., Ironside, C., Cooper, J. M., and Yin, H. |
| College/School: | College of Science and Engineering > School of Engineering College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Lab on a Chip |
| Publisher: | Royal Society of Chemistry |
| ISSN: | 1473-0197 |
| ISSN (Online): | 1473-0189 |
| Copyright Holders: | Copyright © 2015 The Authors |
| First Published: | First published in Lab on a Chip 15(1):283-289 |
| Publisher Policy: | Reproduced under a Creative Commons License |
| Related URLs: |
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