Fan, W. H., Robertson, A. P.E., McElwee, M., Bhella, D. , Rixon, F. J. and Lauder, R. (2015) The large tegument protein pUL36 is essential for formation of the capsid vertex-specific component at the capsid-tegument interface of herpes simplex virus 1. Journal of Virology, 89(3), pp. 1502-1511. (doi: 10.1128/JVI.02887-14) (PMID:25410861) (PMCID:PMC4300765)
|
Text
102623.pdf - Published Version Available under License Creative Commons Attribution. 2MB |
Abstract
Herpesviruses have a characteristic particle structure comprising an icosahedral capsid, which contains the DNA genome and is, in turn, surrounded by a proteinaceous tegument layer and a lipid envelope. In herpes simplex virus, the interaction between the capsid and tegument is limited to the capsid vertices and involves two minor capsid proteins, pUL17 and pUL25, and the large inner tegument protein pUL36. pUL17 and pUL25 form a heterodimeric structure, the capsid vertex-specific component (CVSC), that lies on top of the peripentonal triplexes, while pUL36 has been reported to connect the CVSC to the penton. In this study, we used virus mutants with deletions in the genes for pUL36 and another inner tegument protein, pUL37, to analyze the contributions of these proteins to CVSC structure. Using electron cryomicroscopy and icosahedral reconstruction of mutants that express pUL17 and pUL25 but not pUL36, we showed that in contrast to accepted models, the CVSC is not formed from pUL17 and pUL25 on their own but requires a contribution from pUL36. In addition, the presence of full-length pUL36 results in weak density that extends the CVSC toward the penton, suggesting either that this extra density is formed directly by pUL36 or that pUL36 stabilizes other components of the vertex-tegument interface.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Rixon, Dr Frazer and Bhella, Professor David and McElwee, Dr Marion and Lauder, Dr Rebecca |
Authors: | Fan, W. H., Robertson, A. P.E., McElwee, M., Bhella, D., Rixon, F. J., and Lauder, R. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Journal of Virology |
Publisher: | American Society for Microbiology |
ISSN: | 0022-538X |
ISSN (Online): | 1098-5514 |
Copyright Holders: | Copyright © 2015 The Authors |
First Published: | First published in the Journal of Virology 89(3):1502-1511 |
Publisher Policy: | Reproduced under a Creative Commons License |
Related URLs: |
University Staff: Request a correction | Enlighten Editors: Update this record