Haggarty, J., Oppermann, M., Dalby, M. J. , Burchmore, R. J. , Cook, K., Weidt, S. and Burgess, K. E.V. (2015) Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome. Metabolomics, 11(5), pp. 1465-1470. (doi: 10.1007/s11306-014-0770-7) (PMID:26366140) (PMCID:PMC4559102)
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Abstract
The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-polar metabolites extracted from beer were analysed using a combined RPLC/HILIC method. Non-polar metabolites were retained by the RPLC column. Polar metabolites not retained by the RPLC column were retained and separated by the HILIC column. The results from this study validate this simple yet powerful metabolomics approach.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Weidt, Dr Stefan and Dalby, Professor Matthew and Burchmore, Dr Richard and Burgess, Dr Karl and Haggarty, Ms Jennifer |
Authors: | Haggarty, J., Oppermann, M., Dalby, M. J., Burchmore, R. J., Cook, K., Weidt, S., and Burgess, K. E.V. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Metabolomics |
Publisher: | Springer US |
ISSN: | 1573-3882 |
ISSN (Online): | 1573-3890 |
Copyright Holders: | Copyright © 2015 The Authors |
First Published: | First published in Metabolomics 11(5): 1465-1470 |
Publisher Policy: | Reproduced under a Creative Commons License |
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