Promotor and enhancer like activity at the 5'-end of normal and T24 Ha-ras1 genes

Spandidos, D. A. and Riggio, M. (1986) Promotor and enhancer like activity at the 5'-end of normal and T24 Ha-ras1 genes. FEBS Letters, 203(2), pp. 169-174. (doi: 10.1016/0014-5793(86)80736-0)

Abstract

We have used a short-term transfection technique, in which we monitor the ability of DNA fragments to induce the expression of the chloramphenicol acetyltransferase (CAT) gene in rat 208F fibroblast cells. Using appropriate vectors we have assayed for promoter or enhancer activity of the 0.8 kb SstI fragment located within the 5'-flanking sequences of the first coding exon of the human T24 and normal Ha-ras1 genes. We find that this fragment contains promoter and enhancer activities in both the normal and the T24 Ha-ras1 gene.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Riggio, Professor Marcello
• Authors: Spandidos, D. A., and Riggio, M.
• Subjects: R Medicine > R Medicine (General)
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
• Journal Name: FEBS Letters
• Publisher: Elsevier B.V.
• ISSN: 0014-5793
• ISSN (Online): 1873-3468