Capewell, P. , Cooper, A., Clucas, C. , Weir, W. , Vaikkinen, H., Morrison, L., Tait, A. and MacLeod, A. (2015) Exploiting genetic variation to discover genes involved in important disease phenotypes. Methods in Molecular Biology, 1201, pp. 91-107. (doi: 10.1007/978-1-4939-1438-8_5) (PMID:25388109)
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Abstract
Elucidating the underlying genetic determinants of disease pathology is still in the early stages for many pathogenic parasites. There have, however, been a number of advances in which natural genetic diversity has been successfully utilized to untangle the often complex interactions between parasite and host. In this chapter we discuss various methods capable of exploiting this natural genetic variation to determine genes involved in phenotypes of interest, using virulence in the pathogenic parasite Trypanosoma brucei as a case study. This species is an ideal system to benefit from such an approach as there are several well-characterized laboratory strains; the parasite undergoes genetic exchange in both the field and the laboratory, and is amenable to efficient reverse genetics and RNAi.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Cooper, Dr Anneli and MacLeod, Professor Annette and Morrison, Dr Liam and Tait, Professor Andy and Weir, Professor Willie and O'Connell, Dr Caroline and Capewell, Dr Paul |
Authors: | Capewell, P., Cooper, A., Clucas, C., Weir, W., Vaikkinen, H., Morrison, L., Tait, A., and MacLeod, A. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Methods in Molecular Biology |
Publisher: | Springer New York |
ISSN: | 1064-3745 |
ISSN (Online): | 1940-6029 |
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