Benevento, M., Tonge, P. D., Puri, M. C., Hussein, S. M.I., Cloonan, N., Wood, D. L., Grimmond, S. M., Nagy, A., Munoz, J. and Heck, A. J.R. (2014) Proteome adaptation in cell reprogramming proceeds via distinct transcriptional networks. Nature Communications, 5, (doi: 10.1038/ncomms6613)
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Abstract
The ectopic expression of Oct4, Klf4, c-Myc and Sox2 (OKMS) transcription factors allows reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). The reprogramming process, which involves a complex network of molecular events, is not yet fully characterized. Here we perform a quantitative mass spectrometry-based analysis to probe in-depth dynamic proteome changes during somatic cell reprogramming. Our data reveal defined waves of proteome resetting, with the first wave occurring 48 h after the activation of the reprogramming transgenes and involving specific biological processes linked to the c-Myc transcriptional network. A second wave of proteome reorganization occurs in a later stage of reprogramming, where we characterize the proteome of two distinct pluripotent cellular populations. In addition, the overlay of our proteome resource with parallel generated -omics data is explored to identify post-transcriptionally regulated proteins involved in key steps during reprogramming.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Grimmond, Professor Sean |
Authors: | Benevento, M., Tonge, P. D., Puri, M. C., Hussein, S. M.I., Cloonan, N., Wood, D. L., Grimmond, S. M., Nagy, A., Munoz, J., and Heck, A. J.R. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | Nature Communications |
Publisher: | Nature Publishing Group |
ISSN: | 2041-1723 |
ISSN (Online): | 2041-1723 |
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