Proteome adaptation in cell reprogramming proceeds via distinct transcriptional networks

Benevento, M., Tonge, P. D., Puri, M. C., Hussein, S. M.I., Cloonan, N., Wood, D. L., Grimmond, S. M., Nagy, A., Munoz, J. and Heck, A. J.R. (2014) Proteome adaptation in cell reprogramming proceeds via distinct transcriptional networks. Nature Communications, 5, (doi: 10.1038/ncomms6613)

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Abstract

The ectopic expression of ​Oct4, ​Klf4, ​c-Myc and ​Sox2 (OKMS) transcription factors allows reprogramming of somatic cells into induced pluripotent stem cells (iPSCs). The reprogramming process, which involves a complex network of molecular events, is not yet fully characterized. Here we perform a quantitative mass spectrometry-based analysis to probe in-depth dynamic proteome changes during somatic cell reprogramming. Our data reveal defined waves of proteome resetting, with the first wave occurring 48 h after the activation of the reprogramming transgenes and involving specific biological processes linked to the ​c-Myc transcriptional network. A second wave of proteome reorganization occurs in a later stage of reprogramming, where we characterize the proteome of two distinct pluripotent cellular populations. In addition, the overlay of our proteome resource with parallel generated -omics data is explored to identify post-transcriptionally regulated proteins involved in key steps during reprogramming.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Grimmond, Professor Sean
Authors: Benevento, M., Tonge, P. D., Puri, M. C., Hussein, S. M.I., Cloonan, N., Wood, D. L., Grimmond, S. M., Nagy, A., Munoz, J., and Heck, A. J.R.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Nature Communications
Publisher:Nature Publishing Group
ISSN:2041-1723
ISSN (Online):2041-1723

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