Braceland, M., McLoughlin, M.F., Tinsley, J., Wallace, C., Cockerill, D., McLaughlin, M. and Eckersall, P.D. (2015) Serum enolase: a non-destructive biomarker of white skeletal myopathy during pancreas disease (PD) in Atlantic salmon Salmo salar L. Journal of Fish Diseases, 28(9), pp. 821-831. (doi: 10.1111/jfd.12296) (PMID:25168106)
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Abstract
Diseases which cause skeletal muscle myopathy are some of the most economically damaging diseases in Atlantic salmon, Salmo salar L., aquaculture. Despite this, there are limited means of assessing fish health non-destructively. Previous investigation of the serum proteome of Atlantic salmon, Salmo salar L., during pancreas disease (PD) has identified proteins in serum that have potential as biomarkers of the disease. Amongst these proteins, the enzyme enolase was selected as the most viable for use as a biomarker of muscle myopathy associated with PD. Western blot and immunoassay (ELISA) validated enolase as a biomarker for PD, whilst immunohistochemistry identified white muscle as the source of enolase. Enolase was shown to be a specific marker for white muscle myopathy in salmon, rising in serum concentration significantly correlating with pathological damage to the tissue.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Eckersall, Professor David and McLaughlin, Dr Mark and Braceland, Mr Mark |
| Authors: | Braceland, M., McLoughlin, M.F., Tinsley, J., Wallace, C., Cockerill, D., McLaughlin, M., and Eckersall, P.D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Journal of Fish Diseases |
| Publisher: | Wiley |
| ISSN: | 0140-7775 |
| ISSN (Online): | 1365-2761 |
| Published Online: | 29 August 2014 |
| Copyright Holders: | Copyright © 2014 John Wiley & Sons Ltd. |
| First Published: | First published in the Journal of Fish Diseases 38(9):821-831 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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