McFarlane, M. et al. (2020) The Aedes aegypti Domino orthologue p400 regulates antiviral exogenous small interfering RNA pathway activity and ago-2 expression. mSphere, 5(2), e00081-20. (doi: 10.1128/mSphere.00081-20) (PMID:32269152) (PMCID:PMC7142294)
|
Text
213099.pdf - Published Version Available under License Creative Commons Attribution. 1MB |
Abstract
Arboviruses are pathogens of humans and animals. A better understanding of the interactions between these pathogens and the arthropod vectors, such as mosquitoes, that transmit them is necessary to develop novel control measures. A major antiviral pathway in the mosquito vector is the exogenous small interfering RNA (exo-siRNA) pathway, which is induced by arbovirus-derived double-stranded RNA in infected cells. Although recent work has shown the key role played by Argonaute-2 (Ago-2) and Dicer-2 (Dcr-2) in this pathway, the regulatory mechanisms that govern these pathways have not been studied in mosquitoes. Here, we show that the Domino ortholog p400 has antiviral activity against the alphavirus Semliki Forest virus (Togaviridae) both in Aedes aegypti-derived cells and in vivo. Antiviral activity of p400 was also demonstrated against chikungunya virus (Togaviridae) and Bunyamwera virus (Peribunyaviridae) but not Zika virus (Flaviviridae). p400 was found to be expressed across mosquito tissues and regulated ago-2 but not dcr-2 transcript levels in A. aegypti mosquitoes. These findings provide novel insights into the regulation of an important aedine exo-siRNA pathway effector protein, Ago-2, by the Domino ortholog p400. They add functional insights to previous observations of this protein’s antiviral and RNA interference regulatory activities in Drosophila melanogaster.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Gestuveo, Mr Rommel and Almire, Ms Floriane and Mcdonald, Dr Melanie and Terry, Mrs Sandra and Mcdonald, Mrs Alma and Donald, Dr Claire and Kean, Dr Joy and Loney, Mr Colin and Dietrich, Dr Isabelle and Schnettler, Dr Esther and Varjak, Dr Margus and Pondeville, Dr Emilie and Kohl, Professor Alain |
Creator Roles: | McFarlane, M.Conceptualization, Methodology, Formal analysis, Investigation, Validation, Writing – original draft, Writing – review and editing, Visualization, Data curation Almire, F.Conceptualization, Methodology, Formal analysis, Investigation, Validation, Writing – original draft, Writing – review and editing, Visualization, Data curation Schnettler, E.Conceptualization, Methodology, Formal analysis, Funding acquisition, Writing – review and editing, Supervision, Project administration, Funding acquisition Pondeville, E.Conceptualization, Methodology, Formal analysis, Funding acquisition, Investigation, Validation, Writing – original draft, Writing – review and editing, Visualization, Data curation, Supervision, Project administration, Funding acquisition Kohl, A.Conceptualization, Methodology, Formal analysis, Funding acquisition, Writing – original draft, Writing – review and editing, Visualization, Data curation, Supervision, Project administration, Funding acquisition Donald, C.Methodology, Investigation, Validation, Writing – review and editing Varjak, M.Methodology, Formal analysis, Investigation, Validation, Writing – review and editing Kean, J.Investigation, Validation, Writing – review and editing Mcdonald, A.Investigation, Validation, Writing – review and editing Terry, S.Investigation Gestuveo, R.Investigation Loney, C.Investigation Dietrich, I.Resources |
Authors: | McFarlane, M., Almire, F., Kean, J., Donald, C. L., McDonald, A., Wee, B., Lauréti, M., Varjak, M., Terry, S., Vazeille, M., Gestuveo, R. J., Dietrich, I., Loney, C., Failloux, A.-B., Schnettler, E., Pondeville, E., and Kohl, A. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | mSphere |
Publisher: | American Society for Microbiology |
ISSN: | 2379-5042 |
ISSN (Online): | 2379-5042 |
Copyright Holders: | Copyright © 2020 McFarlane et al. |
First Published: | First published in mSphere 5(2):e00081-20 |
Publisher Policy: | Reproduced under a Creative Commons licence |
Data DOI: | 10.5525/gla.researchdata.705 |
University Staff: Request a correction | Enlighten Editors: Update this record