In vitro evidence consistent with an interaction between wild type and a mutant SOD 1 protein associated with canine degenerative myelopathy

Qi, Y., Montague, P., Loney, C. , Campbell, C., Shafie, I., Anderson, T. and McLaughlin, M. (2019) In vitro evidence consistent with an interaction between wild type and a mutant SOD 1 protein associated with canine degenerative myelopathy. European Journal of Neuroscience, 50(12), pp. 3896-3905. (doi: 10.1111/ejn.14526) (PMID:31336405)

Abstract

Canine degenerative myelopathy (DM) is a progressive neurological disorder that maybe considered to be a large animal model for specific forms of the fatal human disease, familial Amytrophic Lateral Sclerosis (fALS). DM is associated with a c118G>A mutation of the superoxide dismutase 1 (Sod1) gene and a significant proportion of cases are inherited in an autosomal recessive manner in contrast to the largely, but not exclusively, dominant mode of inheritance in fALS. The consensus view is that these Sod1/SOD1 mutations results in a toxic gain of function but the mechanisms remain unclear. Here we used an in vitro neuroblastoma cell line transfection system to monitor wild type and mutant forms of SOD1 fusion proteins containing either a Cherry or an enhanced green fluorescent protein (EGFP) tag. These fusion proteins retained SOD1 enzymatic activity on a native gel assay system. We demonstrate that SOD1 aggregate density is significantly higher in DM transfectants compared to wild type and show by co‐immunoprecipitation and confocal microscopy, evidence for a potential interaction between wild type and mutant forms of SOD1 in co‐transfected cells. While in vitro studies have shown SOD1 heterodimer formation in fALS models, this is the first report for DM SOD1. Therefore, despite for the majority of cases there is a difference in the mode of inheritance between fALS and DM, a similar interaction between wild type and mutant SOD1 forms can occur. Clarifying the role of SOD1 in DM may also be of benefit to understanding the role of SOD1 in fALS.

• Item Type: Articles
• Additional Information: This work was funded by the Ronald Miller Scholarship, School of Veterinary Medicine, University of Glasgow.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McLaughlin, Dr Mark and Shafie, Mrs Intan and Campbell, Ms Clare and Loney, Mr Colin and Montague, Dr Paul and Anderson, Professor Jim
• Authors: Qi, Y., Montague, P., Loney, C., Campbell, C., Shafie, I., Anderson, T., and McLaughlin, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine; College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: European Journal of Neuroscience
• Publisher: Wiley
• ISSN: 0953-816X
• ISSN (Online): 1460-9568
• Published Online: 23 July 2019
• Copyright Holders: Copyright © 2019 John Wiley & Sons
• First Published: First published in European Journal of Neuroscience 50(12):3896-3905
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher