Dunlop, J. I. et al. (2018) Development of reverse genetics systems and investigation of host response antagonism and reassortment potential for Cache Valley and Kairi viruses, two emerging orthobunyaviruses of the Americas. PLoS Neglected Tropical Diseases, 12(10), e0006884. (doi: 10.1371/journal.pntd.0006884) (PMID:30372452) (PMCID:PMC6245839)
|
Text
172298.pdf - Published Version Available under License Creative Commons Attribution. 2MB |
Abstract
Orthobunyaviruses such as Cache Valley virus (CVV) and Kairi virus (KRIV) are important animal pathogens. Periodic outbreaks of CVV have resulted in the significant loss of lambs on North American farms, whilst KRIV has mainly been detected in South and Central America with little overlap in geographical range. Vaccines or treatments for these viruses are unavailable. One approach to develop novel vaccine candidates is based on the use of reverse genetics to produce attenuated viruses that elicit immune responses but cannot revert to full virulence. The full genomes of both viruses were sequenced to obtain up to date genome sequence information. Following sequencing, minigenome systems and reverse genetics systems for both CVV and KRIV were developed. Both CVV and KRIV showed a wide in vitro cell host range, with BHK-21 cells a suitable host cell line for virus propagation and titration. To develop attenuated viruses, the open reading frames of the NSs proteins were disrupted. The recombinant viruses with no NSs protein expression induced the production of type I interferon (IFN), indicating that for both viruses NSs functions as an IFN antagonist and that such attenuated viruses could form the basis for attenuated viral vaccines. To assess the potential for reassortment between CVV and KRIV, which could be relevant during vaccination campaigns in areas of overlap, we attempted to produce M segment reassortants by reverse genetics. We were unable to obtain such viruses, suggesting that it is an unlikely event.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Mair, Mr Daniel and Tong, Dr Lily and Elliott, Professor Richard and Szemiel, Dr Agnieszka and Wilkie, Dr Gavin and Da Silva Filipe, Dr Ana and Navarro, Dr Aitor and Brennan, Dr Benjamin and Hughes, Dr Joseph and Dunlop, Dr James and Vattipally, Dr Sreenu and Li, Dr Ping and Modha, Ms Sejal and Kohl, Professor Alain |
Creator Roles: | Dunlop, J. I.Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review and editing Szemiel, A. M.Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review and editing Navarro, A.Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review and editing Wilkie, G. S.Investigation, Methodology, Resources, Writing – review and editing Tong, L.Investigation, Methodology, Resources Modha, S.Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Writing – review and editing Mair, D.Investigation, Methodology, Resources Sreenu, V. B.Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Writing – review and editing Da Silva Filipe, A.Investigation, Methodology, Resources, Writing – review and editing Li, P.Methodology Brennan, B.Investigation Hughes, J.Formal analysis, Investigation, Methodology, Resources, Software, Visualization, Writing – review and editing Elliott, R. M.Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision Kohl, A.Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review and editing |
Authors: | Dunlop, J. I., Szemiel, A. M., Navarro, A., Wilkie, G. S., Tong, L., Modha, S., Mair, D., Sreenu, V. B., Da Silva Filipe, A., Li, P., Huang, Y.-J. S., Brennan, B., Hughes, J., Vanlandingham, D. L., Higgs, S., Elliott, R. M., and Kohl, A. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | PLoS Neglected Tropical Diseases |
Publisher: | Public Library of Science |
ISSN: | 1935-2727 |
ISSN (Online): | 1935-2735 |
Copyright Holders: | Copyright © 2018 Dunlop et al. |
First Published: | First published in PLoS Neglected Tropical Diseases 12(10): e0006884 |
Publisher Policy: | Reproduced under a Creative Commons License |
Data DOI: | 10.5525/gla.researchdata.599 |
University Staff: Request a correction | Enlighten Editors: Update this record