Nucleotide sequence Of the feline Bmi-1 coding region

Kinnon, S. and Fulton, R. (1999) Nucleotide sequence Of the feline Bmi-1 coding region. DNA Sequence: The Journal of DNA Sequencing and Mapping, 10(4-5), pp. 335-338. (doi: 10.3109/10425179909033960)

Abstract

The bmi-1 gene was discovered as a clonal integration site of the Moloney murine leukaemia virus in B-cell lymphomas. The Bmi-1 protein contains the RING finger motif and is homologous to two Drosophila proteins known to be part of a multimeric protein complex involved in repressing gene transcription. A similar role for the highly conserved Bmi-1 protein in mammalian cells has been suggested. The coding regions for the mouse and human genes are known and are 92% homologous. This study involved PCR amplification, cloning, and sequencing of the 980bp feline bmi-1 coding region which was shown to be 92% and 97% homologous to the mouse and human genes respectively. From the open reading frame the feline protein is 326 amino acids in length and is 99% homologous to the human protein and 97% homologous to the mouse protein. This data is consistent with the closer relationship between the feline and human genomes and provides another experimental system in which to analyse Bmi-1 function.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Fulton, Dr Ruth
• Authors: Kinnon, S., and Fulton, R.
• College/School: College of Medical Veterinary and Life Sciences > School of Life Sciences
• Journal Name: DNA Sequence: The Journal of DNA Sequencing and Mapping
• Publisher: Taylor and Francis
• ISSN: 1042-5179
• ISSN (Online): 1029-2365