Kinnon, S. and Fulton, R. (1999) Nucleotide sequence Of the feline Bmi-1 coding region. DNA Sequence: The Journal of DNA Sequencing and Mapping, 10(4-5), pp. 335-338. (doi: 10.3109/10425179909033960)
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Publisher's URL: http://dx.doi.org/10.3109/10425179909033960
Abstract
The bmi-1 gene was discovered as a clonal integration site of the Moloney murine leukaemia virus in B-cell lymphomas. The Bmi-1 protein contains the RING finger motif and is homologous to two Drosophila proteins known to be part of a multimeric protein complex involved in repressing gene transcription. A similar role for the highly conserved Bmi-1 protein in mammalian cells has been suggested. The coding regions for the mouse and human genes are known and are 92% homologous. This study involved PCR amplification, cloning, and sequencing of the 980bp feline bmi-1 coding region which was shown to be 92% and 97% homologous to the mouse and human genes respectively. From the open reading frame the feline protein is 326 amino acids in length and is 99% homologous to the human protein and 97% homologous to the mouse protein. This data is consistent with the closer relationship between the feline and human genomes and provides another experimental system in which to analyse Bmi-1 function.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Fulton, Dr Ruth |
Authors: | Kinnon, S., and Fulton, R. |
College/School: | College of Medical Veterinary and Life Sciences > School of Life Sciences |
Journal Name: | DNA Sequence: The Journal of DNA Sequencing and Mapping |
Publisher: | Taylor and Francis |
ISSN: | 1042-5179 |
ISSN (Online): | 1029-2365 |
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