Molecular analysis of tumours from feline immunodeficiency virus (FIV)-infected cats: an indirect role for FIV?

Terry, A., Callanan, J. J., Fulton, R., Jarrett, O. and Neil, J. C. (1995) Molecular analysis of tumours from feline immunodeficiency virus (FIV)-infected cats: an indirect role for FIV? International Journal of Cancer, 61(2), pp. 227-232. (doi: 10.1002/ijc.2910610215)

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Publisher's URL: http://dx.doi.org/10.1002/ijc.2910610215

Abstract

Five tumours, which arose in cats naturally or experimentally infected with feline immunodeficiency virus (FIV), were examined with molecular probes to establish tumour cell lineage and to screen for integrated viral sequences. Three of the tumours were classed as B-cell lymphomas on the basis of morphology, immunocytochemistry, rearrangement of immunoglobulin heavy chain genes and lack of rearrangement of T-cell receptor (TCR) β-chain genes. Two of these B-cell tumours arose in specific pathogen-free (SPF) cats experimentally infected with FIV. One case of multi-centric lymphosarcoma came from a cat naturally infected with both FIV and feline leukaemia virus (FeLV). This tumour contained integrated FeLV proviral sequences and was judged to be of T-cell origin on the basis of TCR gene rearrangement. The fifth case was a mast cell tumour. Rearrangement of the c-myc locus was not found in any of the FIV-associated tumours but was shown to be present in a rare immunoblastic B-cell lymphoma which arose in an uninfected SPF cat. None of the FIV-associated tumours showed evidence of integrated FIV sequences by Southern biot hybridisation, despite isolation of infectious virus from in vitro cultures of tumour cells in I case. These results confirm that FIV-associated tumours can occur in the absence of FeLV and suggest that the role of FIV in lymphomagenesis is generally indirect.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Fulton, Dr Ruth and Neil, Professor James and Terry, Mrs Anne
Authors: Terry, A., Callanan, J. J., Fulton, R., Jarrett, O., and Neil, J. C.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:International Journal of Cancer
Publisher:John Wiley and Sons
ISSN:0020-7136
ISSN (Online):1097-0215

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