Orange juice (poly)phenols are highly bioavailable in humans

Pereira-Caro, G., Borges, G., Van Der Hooft, J. , Clifford, M. N., Del Rio, D., Lean, M. E.J. , Roberts, S. A., Kellerhals, M. B. and Crozier, A. (2014) Orange juice (poly)phenols are highly bioavailable in humans. American Journal of Clinical Nutrition, 100(5), pp. 1378-1384. (doi:10.3945/ajcn.114.090282)

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Abstract

Background: We assessed the bioavailability of orange juice (poly)phenols by monitoring urinary flavanone metabolites and ring fission catabolites produced by the action of the colonic microbiota.<p></p> Objective: Our objective was to identify and quantify metabolites and catabolites excreted in urine 0–24 h after the acute ingestion of a (poly)phenol-rich orange juice by 12 volunteers.<p></p> Design: Twelve volunteers [6 men and 6 women; body mass index (in kg/m2): 23.9–37.2] consumed a low (poly)phenol diet for 2 d before first drinking 250 mL pulp-enriched orange juice, which contained 584 μmol (poly)phenols of which 537 μmol were flavanones, and after a 2-wk washout, the procedure was repeated, and a placebo drink was consumed. Urine collected for a 24-h period was analyzed qualitatively and quantitatively by using high-performance liquid chromatography–mass spectrometry (HPLC-MS) and gas chromatography–mass spectrometry (GC-MS).<p></p> Results: A total of 14 metabolites were identified and quantified in urine by using HPLC-MS after orange juice intake. Hesperetin-O-glucuronides, naringenin-O-glucuronides, and hesperetin-3′-O-sulfate were the main metabolites. The overall urinary excretion of flavanone metabolites corresponded to 16% of the intake of 584 μmol (poly)phenols. The GC-MS analysis revealed that 8 urinary catabolites were also excreted in significantly higher quantities after orange juice consumption. These catabolites were 3-(3′-methoxy-4′-hydroxyphenyl)propionic acid, 3-(3′-hydroxy-4′-methoxyphenyl)propionic acid, 3-(3′-hydroxy-4′-methoxyphenyl)hydracrylic acid, 3-(3′-hydroxyphenyl)hydracrylic acid, 3′-methoxy-4′-hydroxyphenylacetic acid, hippuric acid, 3′-hydroxyhippuric acid, and 4′-hydroxyhippuric acid. These aromatic acids originated from the colonic microbiota-mediated breakdown of orange juice (poly)phenols and were excreted in amounts equivalent to 88% of (poly)phenol intake. When combined with the 16% excretion of metabolites, this percentage raised the overall urinary excretion to ∼100% of intake.<p></p> Conclusions: When colon-derived phenolic catabolites are included with flavanone glucuronide and sulfate metabolites, orange juice (poly)phenols are much-more bioavailable than previously envisaged. In vitro and ex vivo studies on mechanisms underlying the potential protective effects of orange juice consumption should use in vivo metabolites and catabolites detected in this investigation at physiologic concentrations. The trial was registered at BioMed Central Ltd (www.controlledtrials.com) as ISRCTN04271658.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Crozier, Professor Alan and Borges, Dr Gina and Pereira Caro, Dr Gema and Lean, Professor Michael and Van Der Hooft, Mr Justin
Authors: Pereira-Caro, G., Borges, G., Van Der Hooft, J., Clifford, M. N., Del Rio, D., Lean, M. E.J., Roberts, S. A., Kellerhals, M. B., and Crozier, A.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:American Journal of Clinical Nutrition
Publisher:American Society for Nutrition
ISSN:0002-9165
ISSN (Online):1938-3207
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