Genetic and antigenic characterisation of serotype A FMD viruses from East Africa to select new vaccine strains

Bari, F. D., Parida, S., Tekleghiorghis, T., Dekker, A., Sangula, A., Reeve, R. , Haydon, D. T. , Paton, D. J. and Mahapatra, M. (2014) Genetic and antigenic characterisation of serotype A FMD viruses from East Africa to select new vaccine strains. Vaccine, 32(44), pp. 5794-5800. (doi:10.1016/j.vaccine.2014.08.033) (PMID:25171846) (PMCID:PMC4194315)

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Abstract

Vaccine strain selection for emerging foot-and-mouth disease virus (FMDV) outbreaks in enzootic countries can be addressed through antigenic and genetic characterisation of recently circulating viruses. A total of 56 serotype A FMDVs isolated between 1998 and 2012, from Central, East and North African countries were characterised antigenically by virus neutralisation test using antisera to three existing and four candidate vaccine strains and, genetically by characterising the full capsid sequence data. A Bayesian analysis of the capsid sequence data revealed the viruses to be of either African or Asian topotypes with subdivision of the African topotype viruses into four genotypes (Genotypes I, II, IV and VII). The existing vaccine strains were found to be least cross-reactive (good matches observed for only 5.4–46.4% of the sampled viruses). Three bovine antisera, raised against A-EA-2007, A-EA-1981 and A-EA-1984 viruses, exhibited broad cross-neutralisation, towards more than 85% of the circulating viruses. Of the three vaccines, A-EA-2007 was the best showing more than 90% in-vitro cross-protection, as well as being the most recent amongst the vaccine strains used in this study. It therefore appears antigenically suitable as a vaccine strain to be used in the region in FMD control programmes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Haydon, Professor Daniel and Reeve, Dr Richard and Paton, Professor David
Authors: Bari, F. D., Parida, S., Tekleghiorghis, T., Dekker, A., Sangula, A., Reeve, R., Haydon, D. T., Paton, D. J., and Mahapatra, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Vaccine
Publisher:Elsevier Ltd.
ISSN:0264-410X
ISSN (Online):1873-2518
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Vaccine 32(44):5794-5800
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
507161Improving the quality of FMD (Foot-and-mouth disease) vaccines by understanding the correlation of vaccine-induced protection with humoral and cellular immune responsesRichard ReeveBiotechnology and Biological Sciences Research Council (BBSRC)BB/H009175/1RI BIODIVERSITY ANIMAL HEALTH & COMPMED