The expression of intelectin in sheep goblet cells and upregulation by interleukin-4

French, A., Bethune, J. A., Knight, P., McNeilly, T. N., Wategedera, S., Rhind, S., Miller, H. R.P. and Pemberton, A. D. (2007) The expression of intelectin in sheep goblet cells and upregulation by interleukin-4. Veterinary Immunology and Immunopathology, 120(1-2), pp. 41-46. (doi: 10.1016/j.vetimm.2007.07.014)

Abstract

Upregulation of intelectin (ITLN) transcript and protein has previously been shown in intestinal nematode infections of resistant mice strains with immunolocalisation of protein to goblet cells and paneth cells. In man, intelectin expression has been shown in respiratory tract epithelium, with upregulation occurring in bronchoalveolar lavage fluid of asthmatic individuals. This study describes the expression of intelectin in the respiratory tract of sheep and the immunolocalisation to goblet cells using a novel affinity-purified chicken anti-intelectin peptide antibody. Furthermore we show that when sheep tracheal explants were cultured for 48 h± recombinant sheep IL-4, sheep ITLN transcripts were upregulated compared with controls. Putative roles for intelectin have included an antibacterial role and an alteration of the character of mucus. Our data suggest ITLNs may play an important role in the mucosal response in allergy and parasitic infections.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Knight, Dr Pamela and French, Professor Anne and Rhind, Dr Susan
• Authors: French, A., Bethune, J. A., Knight, P., McNeilly, T. N., Wategedera, S., Rhind, S., Miller, H. R.P., and Pemberton, A. D.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Veterinary Immunology and Immunopathology
• Publisher: Elsevier B.V.
• ISSN: 0165-2427
• ISSN (Online): 1873-2534