Protection by the flavonoids quercetin and luteolin against peroxide or menadione-induced oxidative stress in MC3T3-E1 osteoblast cells

Fatokun, A. A., Tome, M., Smith, R. A., Darlington, L. G. and Stone, T. W. (2015) Protection by the flavonoids quercetin and luteolin against peroxide or menadione-induced oxidative stress in MC3T3-E1 osteoblast cells. Natural Product Research, 29(12), pp. 1127-1132. (doi: 10.1080/14786419.2014.980252) (PMID:25427161)

Abstract

Potential protective effects of the flavonoids quercetin and luteolin have been examined against the oxidative stress of MC3T3-E1 osteoblast-like cells. Although hydrogen peroxide and menadione reduced cell viability, the toxicity was prevented by desferrioxamine or catalase but not superoxide dismutase, suggesting the involvement of hydrogen peroxide in both cases. Quercetin and luteolin reduced the oxidative damage, especially that caused by hydrogen peroxide. When cultures were pre-incubated with quercetin or luteolin, protection was reduced or lost. Protection was also reduced when a 24 h pre-incubation with the flavonoids was followed by exposure to menadione alone. Pretreating cultures with luteolin impaired protection by quercetin, whereas quercetin pretreatment did not affect protection by luteolin. It is concluded that quercetin and luteolin suppress oxidative damage to MC3T3-E1 cells, especially caused by peroxide. The reduction in protection by pretreatment implies a down-regulation of part of the toxic transduction pathway.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Stone, Professor Trevor and Smith, Professor Robert and Darlington, Dr Lynda and Tome, Dr Mercedes and Fatokun, Dr Amos
• Authors: Fatokun, A. A., Tome, M., Smith, R. A., Darlington, L. G., and Stone, T. W.
• College/School: College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience; College of Medical Veterinary and Life Sciences > School of Life Sciences
• Journal Name: Natural Product Research
• Publisher: Taylor and Francis
• ISSN: 1478-6419
• ISSN (Online): 1478-6427

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