Lack of telomerase RNA gene hTERC expression in alternative lengthening of telomeres cells is associated with methylation of the hTERC promoter

Hoare, S.F., Bryce, L.A., Wisman, G.B.A., Burns, S., Going, J.J., van der Zee, A.G.J. and Keith, W.N. (2001) Lack of telomerase RNA gene hTERC expression in alternative lengthening of telomeres cells is associated with methylation of the hTERC promoter. Cancer Research, 61(1), pp. 27-32.

Hoare, S.F., Bryce, L.A., Wisman, G.B.A., Burns, S., Going, J.J., van der Zee, A.G.J. and Keith, W.N. (2001) Lack of telomerase RNA gene hTERC expression in alternative lengthening of telomeres cells is associated with methylation of the hTERC promoter. Cancer Research, 61(1), pp. 27-32.

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Publisher's URL: http://cancerres.aacrjournals.org/cgi/content/full/61/1/27

Abstract

The immortal phenotype of most human cancers is attributable to telomerase expression. However, a number of immortal cell lines and tumors achieve telomere maintenance in the absence of telomerase via alternative mechanisms known as ALT (alternative lengthening of telomeres). Here we show that the promoter of the telomerase RNA gene (hTERC) is methylated in three of five ALT cell lines and is associated with a total absence of hTERC expression in the three lines. Treatment with 5-azacytidine in combination with trichostatin A resulted in partial demethylation of the hTERC promoter and expression of the gene. Partial methylation was detected in tumors (5%) and in immortal cell lines (27%). Cell lines with partial methylation express hTERC. Only in ALT cell lines does there appear to be a strong correlation between hTERC promoter hypermethylation and lack of hTERC expression.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Keith, Professor Nicol and Burns, Mrs Sharon and Hoare, Miss Stacey and Going, Dr James
Authors: Hoare, S.F., Bryce, L.A., Wisman, G.B.A., Burns, S., Going, J.J., van der Zee, A.G.J., and Keith, W.N.
Subjects:R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Q Science > QH Natural history > QH426 Genetics
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Cancer Research
ISSN:0008-5472

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