Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity o

Bahia, D. S., Sartania, N., Ward, R. j., Cavalli, A., Jones, T. L.Z., Druey, K. M. and Milligan, G. (2003) Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity o. Journal of Neurochemistry, 85(5), pp. 1289-1298. (doi:10.1046/j.1471-4159.2003.01769.x)

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Abstract

Agonists stimulated high-affinity GTPase activity in membranes of HEK293 cells following coexpression of the α2A-adrenoceptor and a pertussis toxin-resistant mutant of Go1α. Enzyme kinetic analysis of Vmax and Km failed to detect regulation of the effect of agonist by a GTPase activating protein. This did occur, however, when cells were also transfected to express RGS4. Both elements of a fusion protein in which the N-terminus of RGS4 was linked to the C-terminal tail of the α2A-adrenoceptor were functional, as it was able to provide concerted stimulation and deactivation of the G protein. By contrast, the α2A-adrenoceptor-RGS4 fusion protein stimulated but did not enhance deactivation of a form of Go1α that is resistant to the effects of regulator of G protein signaling (RGS) proteins. Employing this model system, mutation of Asn128 but not Asn88 eliminated detectable GTPase activating protein activity of RGS4 against Go1α. Mutation of all three cysteine residues that are sites of post-translational acylation in RGS4 also eliminated GTPase activating protein activity but this was not achieved by less concerted mutation of these sites. These studies demonstrate that a fusion protein between a G protein-coupled receptor and an RGS protein is fully functional in providing both enhanced guanine nucleotide exchange and GTP hydrolysis of a coexpressed G protein. They also provide a direct means to assess, in mammalian cells, the effects of mutation of the RGS protein on function in circumstances in which the spatial relationship and orientation of the RGS to its target G protein is defined and maintained.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Milligan, Professor Graeme and Ward, Dr Richard and Sartania, Dr Nana
Authors: Bahia, D. S., Sartania, N., Ward, R. j., Cavalli, A., Jones, T. L.Z., Druey, K. M., and Milligan, G.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Journal of Neurochemistry
ISSN:0022-3042
ISSN (Online):1471-4159
Published Online:06 May 2003

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
325011Post transitional modification and the function of regulator of G protien signallingGraeme MilliganBiotechnology and Biological Sciences Research Council (BBSRC)C17789Institute of Neuroscience and Psychology