Mammary stem cells have myoepithelial cell properties

Prater, M. D. et al. (2014) Mammary stem cells have myoepithelial cell properties. Nature Cell Biology, 16(10), pp. 942-950. (doi: 10.1038/ncb3025) (PMID:25173976) (PMCID:PMC4183554)

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Abstract

Contractile myoepithelial cells dominate the basal layer of the mammary epithelium and are considered to be differentiated cells. However, we observe that up to 54% of single basal cells can form colonies when seeded into adherent culture in the presence of agents that disrupt actin–myosin interactions, and on average, 65% of the single-cell-derived basal colonies can repopulate a mammary gland when transplanted in vivo. This indicates that a high proportion of basal myoepithelial cells can give rise to a mammary repopulating unit (MRU). We demonstrate that myoepithelial cells, flow-sorted using two independent myoepithelial-specific reporter strategies, have MRU capacity. Using an inducible lineage-tracing approach we follow the progeny of myoepithelial cells that express α-smooth muscle actin and show that they function as long-lived lineage-restricted stem cells in the virgin state and during pregnancy

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rath, Dr Nicola and Olson, Professor Michael
Authors: Prater, M. D., Petit, V., Russell, I. A., Giraddi, R. R., Shehata, M., Menon, S., Schulte, R., Kalajzic, I., Rath, N., Olson, M. F., Metzger, D., Faraldo, M. M., Deugnier, M.-A., Glukhova, M. A., and Stingl, J.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Nature Cell Biology
Publisher:Nature Publishing Group
ISSN:1465-7392
ISSN (Online):1476-4679
Published Online:31 August 2014

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