Prater, M. D. et al. (2014) Mammary stem cells have myoepithelial cell properties. Nature Cell Biology, 16(10), pp. 942-950. (doi: 10.1038/ncb3025) (PMID:25173976) (PMCID:PMC4183554)
Full text not currently available from Enlighten.
Abstract
Contractile myoepithelial cells dominate the basal layer of the mammary epithelium and are considered to be differentiated cells. However, we observe that up to 54% of single basal cells can form colonies when seeded into adherent culture in the presence of agents that disrupt actin–myosin interactions, and on average, 65% of the single-cell-derived basal colonies can repopulate a mammary gland when transplanted in vivo. This indicates that a high proportion of basal myoepithelial cells can give rise to a mammary repopulating unit (MRU). We demonstrate that myoepithelial cells, flow-sorted using two independent myoepithelial-specific reporter strategies, have MRU capacity. Using an inducible lineage-tracing approach we follow the progeny of myoepithelial cells that express α-smooth muscle actin and show that they function as long-lived lineage-restricted stem cells in the virgin state and during pregnancy
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Rath, Dr Nicola and Olson, Professor Michael |
Authors: | Prater, M. D., Petit, V., Russell, I. A., Giraddi, R. R., Shehata, M., Menon, S., Schulte, R., Kalajzic, I., Rath, N., Olson, M. F., Metzger, D., Faraldo, M. M., Deugnier, M.-A., Glukhova, M. A., and Stingl, J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | Nature Cell Biology |
Publisher: | Nature Publishing Group |
ISSN: | 1465-7392 |
ISSN (Online): | 1476-4679 |
Published Online: | 31 August 2014 |
University Staff: Request a correction | Enlighten Editors: Update this record