Caloric restriction reveals a metabolomic and lipidomic signature in liver of male mice

Jové, M., Naudí, A., Ramírez-Núñez, O., Portero-Otín, M., Selman, C. , Withers, D. J. and Pamplona, R. (2014) Caloric restriction reveals a metabolomic and lipidomic signature in liver of male mice. Aging Cell, 13(5), pp. 828-837. (doi: 10.1111/acel.12241)

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Abstract

Lipid composition, particularly membrane unsaturation, has been proposed as being a lifespan determinant, but it is currently unknown whether caloric restriction (CR), an accepted life-extending intervention, affects cellular lipid profiles. In this study, we employ a liquid chromatography quadrupole time-of-flight-based methodology to demonstrate that CR in the liver of male C57BL/6 mice: (i) induces marked changes in the cellular lipidome, (ii) specifically reduces levels of a phospholipid peroxidation product, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphatidylcholine, (iii) alters cellular phosphoethanolamine and triglyceride distributional profiles, (iv) affects mitochondrial electron transport chain complexes, increasing complex II and decreasing complex III and (v) is associated with specific changes in liver metabolic pathways. These data demonstrate that CR induces a specific lipidome and metabolome reprogramming event in mouse liver which is associated with lower protein oxidative damage, as assessed by mass spectrometry-based measurements. Such changes may be critical to the increased lifespan and healthspan observed in C57BL/6 mice following CR.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Selman, Professor Colin
Authors: Jové, M., Naudí, A., Ramírez-Núñez, O., Portero-Otín, M., Selman, C., Withers, D. J., and Pamplona, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Aging Cell
Publisher:Wiley-Blackwell Publishing Ltd.
ISSN:1474-9718
ISSN (Online):1474-9726
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Aging Cell 13(5):828-837
Publisher Policy:Reproduced under a Creative Commons License
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