Label-free microfluidic enrichment of ring-stage Plasmodium falciparum-infected red blood cells using non-inertial hydrodynamic lift

Geislinger, T. M., Chan, S., Moll, K., Wixforth, A., Wahlgren, M. and Franke, T. (2014) Label-free microfluidic enrichment of ring-stage Plasmodium falciparum-infected red blood cells using non-inertial hydrodynamic lift. Malaria Journal, 13(1), p. 375. (doi:10.1186/1475-2875-13-375)

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Publisher's URL: http://dx.doi.org/10.1186/1475-2875-13-375

Abstract

Background Understanding of malaria pathogenesis caused by Plasmodium falciparum has been greatly deepened since the introduction of in vitro culture system, but the lack of a method to enrich ring-stage parasites remains a technical challenge. Here, a novel way to enrich red blood cells containing parasites in the early ring stage is described and demonstrated.

Methods A simple, straight polydimethylsiloxane microchannel connected to two syringe pumps for sample injection and two height reservoirs for sample collection is used to enrich red blood cells containing parasites in the early ring stage (8-10 h p.i.). The separation is based on the non-inertial hydrodynamic lift effect, a repulsive cell-wall interaction that enables continuous and label-free separation with deformability as intrinsic marker.

Results The possibility to enrich red blood cells containing P. falciparum parasites at ring stage with a throughput of ~12,000 cells per hour and an average enrichment factor of 4.3 ± 0.5 is demonstrated.

Conclusion The method allows for the enrichment of red blood cells early after the invasion by P. falciparumparasites continuously and without any need to label the cells. The approach promises new possibilities to increase the sensitivity of downstream analyses like genomic- or diagnostic tests. The device can be produced as a cheap, disposable chip with mass production technologies and works without expensive peripheral equipment. This makes the approach interesting for the development of new devices for field use in resource poor settings and environments, e.g. with the aim to increase the sensitivity of microscope malaria diagnosis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Franke, Professor Thomas
Authors: Geislinger, T. M., Chan, S., Moll, K., Wixforth, A., Wahlgren, M., and Franke, T.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Malaria Journal
Publisher:BioMed Central
ISSN:1475-2875
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Malaria Journal 13(1):375
Publisher Policy:Reproduced under a Creative Commons License

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