A comparison of the effects of angiotensin II and Bay K 8644 on responses to noradrenaline mediated via postjunctional α1-and α2-adrenoceptors in rabbit isolated blood vessels

Dunn, W.R., Daly, C.J. , McGrath, J.C. and Wilson, V.G. (1991) A comparison of the effects of angiotensin II and Bay K 8644 on responses to noradrenaline mediated via postjunctional α1-and α2-adrenoceptors in rabbit isolated blood vessels. British Journal of Pharmacology, 103(2), pp. 1475-1483. (doi: 10.1111/j.1476-5381.1991.tb09814.x)

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Publisher's URL: http://dx.doi.org/10.1111/j.1476-5381.1991.tb09814.x

Abstract

1 The effects of angiotensin II (AII) and Bay K 8644 on responses to noradrenaline (NA) mediated via postjunctional α1- and/or α2-adrenoceptors have been compared in three isolated venous preparations from the rabbit, the lateral saphenous vein, the left renal vein and the ear vein.<p></p> 2 A similar action of AII and Bay K 8644 was observed only in the lateral saphenous vein; each potentiated responses to NA after isolation of a homogeneous population of postjunctional α2- adrenoceptors. However, even in this preparation the mechanism of action for these agents was not identical. The sensitivity of KCl-induced contraction to changes in extracellular calcium ions (reflecting activation of voltage-dependent Ca2+ channels) was enhanced by Bay K 8644 but reduced by AII.<p></p> 3 AII produced a selective facilitation of responses mediated via postjunctional α2-adrenoceptors. In the lateral saphenous vein it reduced the effectiveness of prazosin and facilitated responses after isolation of α2-adrenoceptors with phenoxybenzamine and rauwolscine. It directly enhanced responses to NA in the ear vein, where only α2-adrenoceptors are involved. In contrast, AII did not influence responses mediated via postjunctional α1-adrenoceptors in the left renal vein (even after the receptor reserve had been removed with phenoxybenzamine) nor the ‘rauwolscine-resistant’ component of responses to NA in the saphenous vein.<p></p> 4 Bay K 8644 enhanced contractile responses to NA mediated both via α2-adrenoceptors, in the lateral saphenous vein, and via α1-adrenoceptors in the left renal vein. Thus, unlike angiotensin II, no preferential effect was apparent.<p></p> 5 Bay K 8644 was inactive against responses to NA in the rabbit isolated ear vein. Since the sustained component of responses to NA in this preparation is dependent upon the influx of extracellular Ca2+, these observations suggest that the influx of Ca2+ stimulated by NA is mediated via receptor-operated (1,4-dihydropyridine-resistant) Ca2+ channels.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McGrath, Professor John and Daly, Dr Craig
Authors: Dunn, W.R., Daly, C.J., McGrath, J.C., and Wilson, V.G.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:British Journal of Pharmacology
Publisher:Wiley
ISSN:0007-1188
ISSN (Online):1476-5381

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