Scanning electron microscopical observation of an osteoblast/osteoclast co-culture on micropatterned orthopaedic ceramics

Halai, M., Ker, A., Meek, R. D., Nadeem, D., Sjostrom, T., Su, B., McNamara, L. E., Dalby, M. J. and Young, P. S. (2014) Scanning electron microscopical observation of an osteoblast/osteoclast co-culture on micropatterned orthopaedic ceramics. Journal of Tissue Engineering, 5, (doi:10.1177/2041731414552114)

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Publisher's URL: http://dx.doi.org/10.1177/2041731414552114

Abstract

In biomaterial engineering, the surface of an implant can influence cell differentiation, adhesion and affinity towards the implant. On contact with an implant, bone marrow–derived mesenchymal stromal cells demonstrate differentiation towards bone forming osteoblasts, which can improve osteointegration. The process of micropatterning has been shown to improve osteointegration in polymers, but there are few reports surrounding ceramics. The purpose of this study was to establish a co-culture of bone marrow–derived mesenchymal stromal cells with osteoclast progenitor cells and to observe the response to micropatterned zirconia toughened alumina ceramics with 30 µm diameter pits. The aim was to establish whether the pits were specifically bioactive towards osteogenesis or were generally bioactive and would also stimulate osteoclastogenesis that could potentially lead to osteolysis. We demonstrate specific bioactivity of micropatterns towards osteogenesis, with more nodule formation and less osteoclastogenesis compared to planar controls. In addition, we found that that macrophage and osteoclast-like cells did not interact with the pits and formed fewer full-size osteoclast-like cells on the pitted surfaces. This may have a role when designing ceramic orthopaedic implants.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Meek, Mr RM and Dalby, Professor Matthew and McNamara, Dr Laura
Authors: Halai, M., Ker, A., Meek, R. D., Nadeem, D., Sjostrom, T., Su, B., McNamara, L. E., Dalby, M. J., and Young, P. S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Tissue Engineering
Publisher:Sage
ISSN:2041-7314
Copyright Holders:Copyright © 2014 The Authors
First Published:First published in Journal of Tissue Engineering 5
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
502891Micro- and nano-patterning of titanium surfaces for optimal osseointegration of orthopaedic implantsMatthew DalbyEngineering & Physical Sciences Research Council (EPSRC)EP/G048703/1RI MOLECULAR CELL & SYSTEMS BIOLOGY