Hepatic and extra-hepatic transcription of inter-α-inhibitor family genes under normal or acute inflammatory conditions in rat

Daveau, M. et al. (1998) Hepatic and extra-hepatic transcription of inter-α-inhibitor family genes under normal or acute inflammatory conditions in rat. Archives of Biochemistry and Biophysics, 350(2), pp. 315-323. (doi: 10.1006/abbi.1997.0515)

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Publisher's URL: http://dx.doi.org/10.1006/abbi.1997.0515

Abstract

The expression and level of the mRNAs for the five genes that code for a set of plasma proteins collectively referred to as the inter-α-inhibitor family have been studied in rat under a normal condition or in the course of a turpentine-induced, systemic inflammation. In healthy rats, all five mRNAs [H1, H2, H3, H4, and α1-microglobulin/bikunin precursor (AMBP)] are expressed primarily in liver and two of them (H2 and H3) are found to a lower extent in brain. Byin situhybridization onto sections of a normal brain, the H3 mRNA has been precisely localized to the hypothalamus, amygdala, pontine area, optic tectum, and cerebellum. By reverse transcriptase-polymerase chain reaction of total RNAs obtained from a panel of organs, low amounts of one or more mRNA(s) could be detected in other locations (e.g., intestine and stomach). Furthermore, the extrahepatic expressions of several of these genes are up- or downregulated at 20 h after the start of a turpentine-induced inflammation. In liver, the contents of H3 and H4 mRNA are upregulated, whereas those of AMBP and H2 are downregulated during the acute phase. This is accounted for by changes in gene transcription, the kinetics of which is gene-specific. This behavior of H1, H2, H3, H4, and AMBP mRNAs in rat liver is in keeping with more limited analyses made at mRNA and/or protein levels in other species (human, pig) suffering from an acute inflammation. Therefore, the inflammation-associated regulation of these five genes that is conserved between species indicates that the inter-α-inhibitor family members are likely to be important partners of the acute phase response.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Olivier, Dr Emmanuel
Authors: Daveau, M., Jean, L., Soury, E., Olivier, E., Masson, S., Lyoumi, S., Chan, P., Hiron, M., Lebreton, J.-P., Husson, A., Jegou, S., Vaudry, H., and Salier, J.-P.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
Journal Name:Archives of Biochemistry and Biophysics
Publisher:Elsevier
ISSN:0003-9861
ISSN (Online):1096-0384
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