Detergents as tools for the purification and classification of lipid rafts

Chamberlain, L. H. (2004) Detergents as tools for the purification and classification of lipid rafts. FEBS Letters, 559(1-3), pp. 1-5. (doi:10.1016/S0014-5793(04)00050-X)

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Publisher's URL: http://dx.doi.org/10.1016/S0014-5793(04)00050-X

Abstract

The relative insolubility of lipid rafts in cold non-ionic detergents is the most widely used method to purify these fascinating membrane domains from intact cells or membranes. Most of what we know about lipid raft function has been derived from experiments utilising detergent insolubility as the basis for raft purification. Recently, a wider range of detergents have been used to purify ‘rafts’, and rafts have been subclassified based on their differential solubility in different detergents. This minireview critically examines the use of detergents as tools for raft isolation and for the subclassification of rafts.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Chamberlain, Dr Luke
Authors: Chamberlain, L. H.
College/School:College of Medical Veterinary and Life Sciences
Journal Name:FEBS Letters
Publisher:Elsevier BV
ISSN:0014-5793
ISSN (Online):1873-3468
Published Online:21 January 2004

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