Novel method for measuring the adhesion energy of vesicles

Gruhn, T., Franke, T., Dimova, R. and Lipowsky, R. (2007) Novel method for measuring the adhesion energy of vesicles. Langmuir, 23(10), pp. 5423-5429. (doi:10.1021/la063123r)

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Publisher's URL: http://dx.doi.org/10.1021/la063123r

Abstract

Adhering vesicles with osmotically stabilized volume are studied with Monte Carlo simulations and optical microscopy. The simulations are used to determine the dependence of the adhesion area on the vesicle volume, the surface area, the bending rigidity, the adhesion energy per membrane area, and the adhesion potential range. The simulation results lead to a simple functional expression that is supplemented by a correction term for gravity effects. The obtained equation provides a new tool to analyze optical microscopy data and, thus, to measure the adhesion energy per area by analyzing the geometry of the adhering vesicle. The method can be applied in the weak and ultra-weak adhesion regime, where the adhesion energy per area is below 10-6 J/m2. By comparing the shapes of adhering vesicles with different reduced volumes, the bending rigidity can be estimated as well. The new approach is applied to experimental data for lipid vesicles on (i) an untreated and (ii) a monolayer-coated glass surface, providing ultra-weak and weak adhesion strength, respectively.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Franke, Professor Thomas
Authors: Gruhn, T., Franke, T., Dimova, R., and Lipowsky, R.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Langmuir
Publisher:American Chemical Society
ISSN:0743-7463
ISSN (Online):1520-5827
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