Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift

Geislinger, T. M. and Franke, T. (2013) Sorting of circulating tumor cells (MV3-melanoma) and red blood cells using non-inertial lift. Biomicrofluidics, 7(4), 044120. (doi:10.1063/1.4818907)

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Publisher's URL: http://dx.doi.org/10.1063/1.4818907

Abstract

We demonstrate the method of non-inertial lift induced cell sorting (NILICS), a continuous, passive, and label-free cell sorting approach in a simple single layer microfluidic device at low Reynolds number flow conditions. In the experiments, we exploit the non-inertial lift effect to sort circulating MV3-melanoma cells from red blood cell suspensions at different hematocrits as high as 9%. We analyze the separation process and the influence of hematocrit and volume flow rates. We achieve sorting efficiencies for MV3-cells up to EMV3 = 100% at Hct = 9% and demonstrate cell viability by recultivation of the sorted cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Franke, Professor Thomas
Authors: Geislinger, T. M., and Franke, T.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Biomicrofluidics
Publisher:American Institute of Physics
ISSN:1932-1058

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