Delivery of non-toxic fragments of Clostridium difficile toxin A to the mucosal immune system

Ward, S. J., Douce, G. , Dougan, G. and Wren, B. W. (1997) Delivery of non-toxic fragments of Clostridium difficile toxin A to the mucosal immune system. Reviews in Medical Microbiology, 8(Sup 1), S34-S36. (doi:10.1097/00013542-199712001-00016)

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Abstract

Polymerase chain reaction fragments coding for 8, 14, 20 and 36 C-terminal repeats from Clostridium difficile toxin A were expressed as fusions to fragment C of tetanus toxin. These fusions were expressed within a rationally attenuated Salmonella typhimurium htrA mutant which has been shown to be effective at delivering heterologous antigens to the gastro-intestinal mucosa. The larger fragments retained their receptor-binding properties, and the construct containing 14 repeats induced an antitoxin A serum response within mice.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Douce, Dr Gillian
Authors: Ward, S. J., Douce, G., Dougan, G., and Wren, B. W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Reviews in Medical Microbiology
Publisher:Lippincott Williams & Wilkins
ISSN:0954-139X
ISSN (Online):1473-5601

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