Mucosal and systemic immunogenicity of a recombinant, non-ADP-ribosylating pertussis toxin: effects of formaldehyde treatment

Cropley, I., Douce, G. , Roberts, M., Chatfield, S., Pizza, M., Marsili, I., Rappuoli, R. and Dougan, G. (1995) Mucosal and systemic immunogenicity of a recombinant, non-ADP-ribosylating pertussis toxin: effects of formaldehyde treatment. Vaccine, 13(17), pp. 1643-1648. (doi: 10.1016/0264-410X(95)00134-M)

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Abstract

The effect of formaldehyde treatment on the mucosal and systemic immunogenicity of the geneticallydetoxified pertussis toxin () was investigated. Groups of were immunized intranasally (i.n.) or subcutaneously (s.c.) with untreated, lightly formaldehyde treated (LFT) or heavily formaldehyde treated (HFT) recombinant pertussis toxin (PT) mutant, . Intranasal immunization with native induced significant levels of anti-toxin antibodies in serum and IgA anti-toxin responses in nasal and lung lavages of these mice. Similar local and systemic responses were observed following intransal immunization with LFT toxin. However, i.n. immunization with HFT toxin failed to induce a local IgA response and elicited a much diminished anti-toxin response in the serum. In contrast, the total antibody response following s.c. immunization was not significantly affected. In addition, i.n. immunization with native induced low but detectable levels of toxin neutralizing antibodies in the serum. These results show that native protein acts as a mucosal immunogen in mice and that this activity is greatly diminished by HFT of the protein.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Douce, Dr Gillian
Authors: Cropley, I., Douce, G., Roberts, M., Chatfield, S., Pizza, M., Marsili, I., Rappuoli, R., and Dougan, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Vaccine
Publisher:Elsevier Ltd.
ISSN:0264-410X
ISSN (Online):1873-2518

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