Influence of codon usage on the immunogenicity of a DNA vaccine against tetanus

Stratford, R., Douce, G. , Zhang-Barber, L., Fairweather, N., Eskola, J. and Dougan, G. (2000) Influence of codon usage on the immunogenicity of a DNA vaccine against tetanus. Vaccine, 19(7-8), pp. 810-815. (doi: 10.1016/S0264-410X(00)00246-2)

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Abstract

Two related DNA vaccine vector plasmids, harbouring either wild-type (pcDNA3/ntetC) or synthetic codon optimised (pcDNA3/stetC) DNA encoding fragment C (TetC) of tetanus toxin were constructed. COS-7 cells transformed with pcDNA3/stetC reproducibly expressed higher levels of TetC than similar cells transformed with pcDNA3/ntetC. BALB/c mice immunised intramuscularly with pcDNA3/stetC produced significantly higher levels of anti-TetC antibodies in their serum in the weeks following vaccination compared to mice immunised with pcDNA3/ntetC, even when differences in the CpG content between the two sequences were controlled for using non-expressing DNA.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Douce, Dr Gillian
Authors: Stratford, R., Douce, G., Zhang-Barber, L., Fairweather, N., Eskola, J., and Dougan, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Vaccine
Publisher:Elsevier Ltd.
ISSN:0264-410X
ISSN (Online):1873-2518

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