The development of oral vaccines based on live attenuated Salmonella strains

Chatfield, S., Roberts, M., Londono, P., Cropley, I., Douce, G. and Dougan, G. (1993) The development of oral vaccines based on live attenuated Salmonella strains. FEMS Immunology and Medical Microbiology, 7(1), pp. 1-7. (doi:10.1111/j.1574-695X.1993.tb00374.x)

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Abstract

Safe, live attenuated Salmonella strains can be produced by introducing defined non-reverting mutations into the chromosome. Such rationally attenuated strains have proved to be excellent oral vaccines in several animal species and can therefore be considered as candidate vaccines against invasive salmonellosis in both animals and man. A panel of attenuating lesions is now available from which it is possible to tailor the level of attenuation and hence produce strains with different immunogenic properties. Because of the spectrum of immune responses produced by such Salmonella vaccine strains they have been utilised extensively as vectors for delivering heterologous antigens to the mammalian immune system. We have focussed on the development of a single dose oral tetanus vaccine based on attenuated Salmonella strains expressing a non-toxic, immunogenic protein derived from tetanus toxin (fragment C). Several different expression systems have been used for fragment C and candidate vaccine strains have been constructed that are capable of protecting orally immunised mice against a lethal challenge with tetanus toxin. An oral tetanus vaccine may help to reduce the mortality rate from tetanus in the developing world by overcoming the problems associated with the implementation of vaccine programmes using the current parenteral vaccine.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Douce, Dr Gillian
Authors: Chatfield, S., Roberts, M., Londono, P., Cropley, I., Douce, G., and Dougan, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:FEMS Immunology and Medical Microbiology
Publisher:Wiley
ISSN:0928-8244
ISSN (Online):2049-632X

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